論文Papers

代表的な論文（簡単な解説付き）Selected papers with notes

1. Bacteria break through one-micrometer-square passages by flagellar wrapping

   Yoshioka A, Shimada YY, Omori T, Uemura NA, Takeshita K, Ishigami K, Morimura H, Furubayashi M, Kan T*, Wada H*, Kikuchi Y*, Nakane D*

   Nature Communications 17: 713 (2026)

   細菌がべん毛を巻き付けて狭小空間を突破することを示しました。菅先生チームがデバイスをつくってくれて、和田さんチームが物理のシミュレーションを、菊池さんチームが昆虫や共生細菌の遺伝学をすすめてくれました。学術変革B 微生物が動く意味のプロジェクトチームのみんなの成果です。中根研1期生の吉岡くんがたくさん実験をしてくれたおかげです。学生の発表賞も 1 2 3 4 ほどいただきました。電通大のニュースリリースや、EurekAlert! に登録し、ブログにも内容を記載しました。

   We showed that bacteria can squeeze through narrow spaces by wrapping their flagella around their cell bodies. Prof. Kan's team built the devices, Wada's team ran the physics simulations, and Kikuchi's team worked on the genetics of the insects and their symbiotic bacteria. This is a result from the whole team of the KAKENHI Transformative Research Areas (B) project "Why Microbes Move". It was made possible by Aoba, a member of the Nakane Lab's first cohort, who did a huge number of experiments. Students also received several presentation awards: 1 2 3 4. We published a UEC news release, posted it on EurekAlert!, and also wrote about it in a blog post.

2. Rapid water flow triggers long-distance positive rheotaxis for thermophilic bacteria

   Uemura NA, Chiba N, Morikawa R, Tamakoshi M, Nakane D*

   The ISME Journal 19: wraf164 (2025)

   温泉などから単離される高度好熱菌 Thermus thermophilus は水流に逆らって運動することを示しました。これを正の走流性と呼びます。栄養分がなくなると、自身の先端を固体表面に付着させたまま立ち上がり、そのまま上流に向かいます。動画はこちら。このような応答はこの細菌に近縁なたくさんの細菌で一般的であることを見出しました。上村くんがいっぱい実験してくれました。学生の発表賞も 1, 2, 3, 4, 5, 6, 7, 8 ほどいただきました。電通大のニュースリリースにも掲載してもらいました。EurekAlert! にも登録しました。Editor's Choice にも選ばれました!

   We showed that the extremely thermophilic bacterium Thermus thermophilus (isolated from places such as hot springs) moves against water flow. This is called positive rheotaxis. When nutrients run out, the cells stand up with one pole still attached to the solid surface and move upstream in that position. Watch the video here. We found that this response is common among many bacteria closely related to this species. Naoki did a lot of the experiments. Students also received several presentation awards: 1, 2, 3, 4, 5, 6, 7, 8. The work was featured in a UEC news release, and we also posted it on EurekAlert!. It was even selected as an Editor's Choice!

3. Cell shape controls rheotaxis in small parasitic bacteria

   Nakane D†*, Kabata Y†, Nishizaka T*

   PLOS Pathogens 18: e1010648 (2022)

   マイコプラズマという寄生性の小さな細菌が、走流性を示すことを見つけました。走流性とは、流れに応答して自身の運動方向を制御させる能力のことを指します。魚などは流れに逆らって泳ぐことが知られていますが、同様の能力を最小の生物であるマイコプラズマも持つようです。マイコプラズマはヒトを含む高等生物に寄生して生活していることが良く知られていますが、それらの実環境中にある水流の流れをうまく利用して、自身の目的地へと到達しているのだと考えられます。学習院大学所属時の学生 加畑くんがたくさん実験してくれました。感謝です。加畑くんは日本生物物理学会年会で、学生発表賞を受賞しています。この論文のプレスリリースはこちら。

   We found that Mycoplasma, a small parasitic bacterium, shows rheotaxis. Rheotaxis is the ability to steer one's direction of movement in response to flow. Fish are known to swim against the current, and it seems that Mycoplasma, the smallest living organism, has a similar ability. Mycoplasma is well known to live as a parasite on higher organisms, including humans, and it probably makes clever use of the fluid flow in these real environments to reach its destination. Kabata, a student in my group when I was at Gakushuin University, did a lot of the experiments. Many thanks. Yoshiki received the Student Presentation Award at the Annual Meeting of the Biophysical Society of Japan. The press release for this paper is here.

4. Thermosynechococcus switches the direction of phototaxis by a c-di-GMP dependent process with high spatial resolution

   Nakane D†*, Enomoto G†*, Bähre H, Hirose Y, Wilde A*, Nishizaka T

   eLife 11: e73405 (2022)

   サーモシネココッカスという好熱性の藍藻において、走光性の正と負の切り替えが c-di-GMP という分子によって制御されていることを示しました。光を受けると藍藻は短軸に沿った細胞極性を生み出し、IV型線毛を非対称に分布させて指向性のある運動を示します。45℃での観察は難しい部分もありましたが、フライブルグにいる榎本さんの変異株が完璧で、たった 7 nm ほどの細さのIV型線毛繊維の動態を可視化することができました。Annegret さんと共にドイツと日本で協力して、素敵な研究成果が得られました。この論文のプレスリリースはこちら。eLife Digest にも選ばれました。Twitter で榎本さんが解説してくれています。

   We showed that in the thermophilic cyanobacterium Thermosynechococcus, switching between positive and negative phototaxis is controlled by a molecule called c-di-GMP. When exposed to light, the cells create a polarity along their short axis and distribute type IV pili asymmetrically, which produces directional movement. Observing at 45 °C was tricky in places, but the mutants made by Gen in Freiburg were perfect, and we were able to visualize the dynamics of type IV pilus filaments only about 7 nm thick. Working together with Annegret across Germany and Japan, we got a wonderful result. The press release for this paper is here. It was also selected for an eLife Digest . Gen explains the work on Twitter.

5. Molecular ruler of the attachment organelle in Mycoplasma pneumoniae

   Nakane D†*, Murata K†, Kenri T†, Shibayama K, Nishizaka T*

   PLOS Pathogens 17: e1009621 (2021)

   ヒト肺炎の病原細菌に「ものさし」として機能するタンパク質があることを見つけました。ものさし分子を人為的に改変して 短く/長く すると、接着器官という 300 nm ほどの超分子構造体を 短く/長く 変化させることが可能です。学習院大学所属時の学生 村田くんと感染研の見理さんがたくさん変異株をつくって実験をしてくれました。感謝です。村田くんは日本細菌学会総会や日本マイコプラズマ学会学術集会で優秀発表賞を受賞しています。この論文のプレスリリースはこちら。

   We found that a pathogenic bacterium that causes human pneumonia has a protein that works as a "ruler". By artificially making this ruler molecule shorter or longer, we can make the attachment organelle, a supramolecular structure about 300 nm long, shorter or longer. Kohki, a student in my group when I was at Gakushuin University, and Tsuyoshi at the National Institute of Infectious Diseases made many mutants and did lots of experiments. Many thanks. Kohki received Excellent Presentation Awards at the Annual Meeting of the Japanese Society for Bacteriology and at the meeting of the Japanese Society of Mycoplasmology. The press release for this paper is here.

6. Large-scale vortices with dynamic rotation emerged from monolayer collective motion of gliding Flavobacteria

   Nakane D*, Odaka S, Suzuki K, Nishizaka T*

   Journal of Bacteriology 203: e00073-21 (2021)

   フラボバクテリウムという細菌が集団になると巨大な渦模様をつくることを見つけました。その渦は、なぜか必ず反時計回りに回転します。土壌にいる細菌ですので、ふと土のなかを覗いてみると、私たちの知らない間にぐるぐると回っているのかもしれませんね。学習院大学所属時の学生 小高さんが主に頑張って実験をしてくれました。Movieなどはこちらをご覧ください。プレスリリースはこちら。

   We found that the bacterium Flavobacterium forms huge swirling patterns when cells gather in groups. For some reason, the swirls always rotate counterclockwise. Since this bacterium lives in soil, if you could peek into the ground, it might be spinning round and round there without us ever knowing. Shoko, a student in my group when I was at Gakushuin University, did most of the hard work on the experiments. See here for movies. The press release is here.

7. Campylobacter jejuni motility integrates specialized cell shape, flagellar filament, and motor, to coordinate action of its opposed flagella

   Cohen EJ†, Nakane D†, Kabata Y, Hendrixson DR, Nishizaka T, Beeby M*

   PLOS Pathogens 16: e1008620 (2020)

   カンピロバクターという病原細菌のユニークな遊泳様式を明らかにしました。2本あるべん毛のうち、片方を体に巻き付けて、ドリルのように進みます。MorganとEli との共同研究。Eliがつくった変異株がバッチリで、べん毛が綺麗に標識されています。Eli はしばらく日本に滞在してくれて、毎日のようにビールを飲みながら研究のことを話していました。良い思い出です。コロナの前のことですので、随分昔のことのように思えます。プレスリリースはこちら。

   We revealed the unique swimming style of the pathogenic bacterium Campylobacter. Of its two flagella, it wraps one around its body and moves forward like a drill. This was a collaboration with Morgan and Eli. The mutants Eli made were spot on, with the flagella beautifully labeled. Eli stayed in Japan for a while, and we talked about research almost every day over beers. Good memories. That was before COVID, so it feels like a long time ago. The press release is here.

8. Co-existence of two chiral helices produces kink translation in Spiroplasma swimming

   Nakane D†*, Ito T†, and Nishizaka T*

   Journal of Bacteriology 202: e00735-19 (2020)

   スピロプラズマという「らせん」細菌は、べん毛がないのに自由に動くことができます。バクテリアの中で、べん毛を使わずに遊泳できるのは、スピロプラズマとシネココッカスのみ。体をねじって、2つのらせんをつくることで、上手に推進力を出すのですが、その伝搬は受動的であることを示しました。学習院大学所属時の学生 伊藤くんが頑張って実験してくれました。この学術誌の表紙にも採択されました。

   Spiroplasma, a helical bacterium, can move freely even though it has no flagella. Among bacteria, only Spiroplasma and Synechococcus are known to swim without flagella. Spiroplasma twists its body to form two helices and generates propulsion very efficiently, and we showed that the propagation of this twist is passive. Tatsuro, a student in my group when I was at Gakushuin University, worked hard on the experiments. The work was also chosen for the cover of the journal.

9. Motor torque measurement of Halobacterium salinarum archaellar suggests a general model for ATP-driven rotary motors

   Iwata S, Kinosita Y, Uchida N, Nakane D*, Nishizaka T*

   Communications Biology 2: 199 (2019)

   ハロバクテリアというアーキア（古細菌）が持つべん毛モーターのトルクを計測しました。実は、アーキアとバクテリアはどちらも「べん毛」という運動装置を持っていますが、全く異なる分子から構成されており進化的起源が異なります。アーキアのべん毛にはまだまだわからないことがたくさんあります。この研究は、学習院所属時の学生 岩田くんが頑張って実験してくれました。そういえば岩田くんは競馬が好きでした。電通大から府中の競馬場は近いですね。プレスリリースはこちら。

   We measured the torque of the flagellar motor of Halobacterium, an archaeon. Archaea and bacteria both have a motility machine called the "flagellum", but the two are made of completely different molecules and have different evolutionary origins. There is still a lot we don't know about archaeal flagella. Seiji, a student in my group when I was at Gakushuin, worked hard on the experiments for this study. Come to think of it, Iwata loved horse racing. The Fuchu racecourse is close to UEC. The press release is here.

10. Asymmetric distribution of type IV pili triggered by directional light in unicellular cyanobacteria

    Nakane D, and Nishizaka T*

    Proc. Natl. Acad. Sci. USA 114: 6593-98 (2017)

    シアノバクテリアが「糸」を使ってスパイダーマンのように動く仕組みを明らかにしました。IV型線毛という細い繊維構造は根元のモーターで伸びたり縮んだりすることができます。しかも、この伸縮を上手に制御して、光に向かって動いたり、逆に、強い光からは逃げるような動きを示します。この仕組みについても言及しています。シアノバクテリアが光の向きを認識する仕組みについては、一歩先にNilsとAnnegretさんたちが報告しました。けれど、その論文も面白いです。 シアノバクテリアの変異株は東工大の増田さんからいただきました。ありがとうございます。この論文は、私と西坂さんだけしか名前がありません。学習院大学 西坂研にうつってから4年経って、しっかりとした成果がでました。とても嬉しかったのを覚えています。プレスリリースはこちら。

    We revealed how cyanobacteria use "threads" to move like Spider-Man. Thin filaments called type IV pili can extend and retract, driven by a motor at their base. What's more, the cells control this extension and retraction cleverly to move toward light, or conversely to move away from strong light. We also discuss how this works. Nils, Annegret and colleagues reported how cyanobacteria sense the direction of light just ahead of us, but their paper is also very interesting. The cyanobacterial mutants were kindly provided by Masuda at Tokyo Tech. Thank you. Only Nishizaka and I are authors on this paper. Four years after moving to the Nishizaka Lab at Gakushuin University, I finally had a solid result. I remember being really happy. The press release is here.

11. Systematic structural analyses of attachment organelle in Mycoplasma pneumoniae

    Nakane D†, Kenri T†, Matsuo L and Miyata M*

    PLOS Pathogens 11: e1005299 (2015)

    ヒト肺炎の病原菌の運動装置がどのようなタンパク質から構成され、ナノレベルで配置しているのか、詳しく調べました。マイコプラズマは泳ぐことはできません。代わりに、宿主やガラスの表面にはりついて動くことができます。これを滑走運動といいます。滑走運動はマイコプラズマの病原性発揮にも重要であると考えられています。何十種類もの変異株を解析しましたが、それらはすべて国立感染症研究所の見理さんがつくってくれました。そういえば、感染研のある武蔵村山も近いですね。見理さん、遊びに来てください。

    We studied in detail which proteins make up the motility machinery of the bacterium that causes human pneumonia, and how they are arranged at the nanoscale. Mycoplasma cannot swim. Instead, it moves while stuck to the surface of host cells or glass. This is called gliding motility, and it is thought to be important for Mycoplasma's ability to cause disease. We analyzed dozens of mutants, all of which were made by Kenri at the National Institute of Infectious Diseases. Come to think of it, Musashimurayama, where the institute is located, is also close by. Kenri, please come and visit us.

12. Unitary step of gliding machinery in Mycoplasma mobile

    Kinosita Y†, Nakane D†, Sugawa M, Masaike T, Mizutani K, Miyata M*, Nishizaka T*

    Proc. Natl. Acad. Sci. USA 111: 8601-06 (2014)

    最小の生物、マイコプラズマが一定距離の推進と停止を繰り返すことで進むことを明らかにしました。その距離はたった 70 nm （ナノメートル）ほどです。最小生物にしては随分と大きな歩幅をもっています。きっと「あし」のようなものを持って動いているのだと思われます。詳しいことは私の出身研究室である大阪市立大学の宮田先生のサイトをみてください。この研究は私が大学院生のときに始めたものですが、学習院大学所属時の学生 木下くんが「ゴースト」という実験系を使って、大きく発展してくれました。彼は育志賞も受賞しました。

    We showed that Mycoplasma, the smallest living organism, moves by repeating cycles of moving a fixed distance and then stopping. That distance is only about 70 nm (nanometers). That's quite a big step for the smallest organism. It probably moves using something like "legs". For details, please see the website of Prof. Miyata at Osaka City University, the lab where I did my graduate work. I started this research as a graduate student, and Kinoshita, a student in my group when I was at Gakushuin University, developed it much further using an experimental system called the "ghost". He also received the JSPS Ikushi Prize.

13. Helical flow of surface protein required for bacterial gliding motility

    Nakane D, Sato K, Wada H, McBride MJ* and Nakayama K*

    Proc. Natl. Acad. Sci. USA 110: 11145-11150 (2013)

    フラボバクテリウムという細菌の動く仕組みを明らかにしました。なんと、自身の表面タンパク質を膜表面でぐるぐると高速で流れるように動かすことで表面を這うように推進しているようです。戦車のキャタピラを彷彿とさせます。これは長崎大学でポスドクをしていたときの仕事です。そのときのボスの中山先生と研究室のみなさんには随分とお世話になりました。変異株や抗体などのリソースは Mark からもらいました。ありがとうございます。彼のラボに2か月くらい滞在したときに、この論文のきっかけとなる成果を得ました。夜に実験したときにうまくいったのですが、あの瞬間は忘れられません。

    We revealed how the bacterium Flavobacterium moves. Remarkably, it seems to crawl over surfaces by making its own surface proteins flow rapidly around and around on the membrane surface. It reminds me of the tracks on a tank. This was work from my postdoc at Nagasaki University. My boss there, Prof. Nakayama, and everyone in the lab took such good care of me. Resources such as mutants and antibodies came from Mark. Thank you. While I stayed in his lab for about two months, I got the result that led to this paper. The experiment finally worked late one night, and I will never forget that moment.

14. Cytoskeletal "jellyfish" structure of Mycoplasma mobile

    Nakane D and Miyata M*

    Proc. Natl. Acad. Sci. USA 104: 19518-19523 (2007)

    マイコプラズマが「くらげ」のような形の細胞骨格を持っていることを明らかにしました。その形だけでなく、その構成タンパク質もユニークです。ATP合成酵素の F1 とよく似たタンパク質からできています。大阪市立大学の宮田研で大学院生をしていたときの論文です。ドキドキしながら海外で口頭発表したのを覚えています。大学院生のときに面白い成果を得ることができたことはとても幸運でした。自分自身が研究者になったのはこのような経験があったからだと思います。自分の研究室ができたので、新しく配属される学生さんにもこのような経験ができるよう、素敵な研究環境を整えたいと思います。

    We showed that Mycoplasma has a cytoskeleton shaped like a "jellyfish". Not only its shape but also its component proteins are unique: they are made of proteins closely resembling F1 of ATP synthase. This paper is from my time as a graduate student in the Miyata Lab at Osaka City University. I remember giving an oral presentation abroad with my heart pounding. I was very lucky to get such an interesting result as a graduate student. I think experiences like this are why I became a researcher. Now that I have my own lab, I want to build a great research environment so that new students joining the lab can have experiences like this too.

Original papers

1. 2026
   Body-wrapping anterior flagella drive ultrafast swimming in bacterial zoospores preprint

   Uemura NA, Ishida T, Sowa Y, Jang MS, Tezuka T*, Nakane D*, Ohnishi Y

   bioRxiv 2026.03.29.715058 (2026)
2. 2026
   Trimeric autotransporter adhesins driving chain-like adhesion diversify surface colonization strategies in Shiga toxin-producing Escherichia coli

   Kotaka Y*, Uemura NA, Iwase T, Lee K, Ishijima N, Nakane D, Kobayashi H, Kataoka M, Akeda Y, Iyoda S

   Nature Communications 17: 9350 (2026)
3. 2026
   Comparison of predatory phenotypes and genotypes between Bdellovibrio sp. BIS2 and Bacteriovorax sp. HI3 isolated from the same freshwater environment

   Sugiyama T, Kojima T, Uemura NA, Nakane D, Dohra H, Inoue D*, Ike M

   Environmental Microbiology 28: e70243 (2026)
4. 2026
   Soil pH as an external filter shaping insect–microbe gut symbiosis

   Itoh H*, Shimoji H, Nakane D, Jang S, Kikuchi Y*

   Microbiome 14: 129 (2026)
5. 2026
   Bacteria harness torque-induced buckling instability for flagellar wrapping

   Kataoka T, Yoneda T, Nakane D, Wada H*

   Physical Review E 113: 054402 (2026)
6. 2026
   A Trojan horse pathogen breaking through partner-choice barriers in the insect gut

   Ishigami K*, Jang S, Yoshioka A, Morimura H, Yokota A, Moulin L, Lirette AO, Takeshita K, Nakane D, Mergaert P, Kikuchi Y*

   Proc. Natl. Acad. Sci. USA 123: e2533244123 (2026)
7. 2026
   Bacteria break through one-micrometer-square passages by flagellar wrapping

   Yoshioka A, Shimada YY, Omori T, Uemura NA, Takeshita K, Ishigami K, Morimura H, Furubayashi M, Kan T*, Wada H*, Kikuchi Y*, Nakane D*

   Nature Communications 17: 713 (2026)
8. 2025
   Rapid water flow triggers long-distance positive rheotaxis for thermophilic bacteria

   Uemura NA, Chiba N, Morikawa R, Tamakoshi M, Nakane D*

   The ISME Journal 19: wraf164 (2025)
9. 2025
   Gliding direction of Mycoplasma mobile correlates with the curved configuration of its cell shape

   Suzuki K, Nakane D, Mizutani M, Nishizaka T*

   Biophysics and Physicobiology, 22: e220006 (2025)
10. 2024
    Evolution of a large periplasmic disk in Campylobacterota flagella facilitated efficient motility alongside autoagglutination

    Cohen EJ*, Drobnič T, Ribardo DA, Yoshioka A, Umrekar T, Guo X, Fernandez J, Brock E, Wilson L, Nakane D, Hendrixson DR, Beeby M

    Developmental Cell 59: 3306-3321.e5 (2024)
11. 2024
    Internal structure of Mycoplasma mobile gliding machinery analyzed by negative staining electron tomography

    Fukushima M, Toyonaga T, Tahara YO, Nakane D, Miyata M*

    Biophysics and Physicobiology 21: e210015 (2024)
12. 2024
    Cell surface architecture of the cultivated DPANN archaeon Nanobdella aerobiophila

    Kato S*, Tahara Y, Nishimura Y, Uematsu K, Arai T, Nakane D, Ihara A, Nishizaka T, Iwasaki W, Itoh T, Makoto M, Ohkuma M

    Journal of Bacteriology 206:e00351-23 (2024)
13. 2023
    Filamentous structures in the cell envelope associated with Bacteroidetes gliding machinery

    Shibata S*, Tahara YO, Katayama E, Kawamoto A, Kato T, Zhu Y, Nakane D, Namba K, Miyata M, McBride MJ, Nakayama K*

    Communications Biology 5: 94 (2023)
14. 2022
    Self-assembled micro-sized hexagons built from short DNA in a crowded environment

    Makino T, Nakane D, Tanaka M*

    ChemBioChem 23:e202200360 (2022)
15. 2022
    Cell shape controls rheotaxis in small parasitic bacteria

    Nakane D†*, Kabata Y†, Nishizaka T*

    PLOS Pathogens 18: e1010648 (2022)
16. 2022
    Thermosynechococcus switches the direction of phototaxis by a c-di-GMP dependent process with high spatial resolution

    Nakane D†*, Enomoto G†*, Bähre H, Hirose Y, Wilde A*, Nishizaka T

    eLife 11: e73405 (2022)
17. 2021
    Molecular ruler of the attachment organelle in Mycoplasma pneumoniae

    Nakane D†*, Murata K†, Kenri T†, Shibayama K, Nishizaka T*

    PLOS Pathogens 17: e1009621 (2021)
18. 2021
    Large-scale vortices with dynamic rotation emerged from monolayer collective motion of gliding Flavobacteria

    Nakane D*, Odaka S, Suzuki S, Nishizaka T*

    Journal of Bacteriology 203: e00073-21 (2021)
19. 2020
    Campylobacter jejuni motility integrates specialized cell shape, flagellar filament, and motor, to coordinate action of its opposed flagella

    Cohen EJ†, Nakane D†, Kabata Y, Hendrixson DR, Nishizaka T, Beeby M*

    PLOS Pathogens 16: 1008620 (2020)
20. 2020
    Co-existence of two chiral helices produces kink translation in Spiroplasma swimming

    Nakane D†*, Ito T†, and Nishizaka T*

    Journal of Bacteriology 202: e00735-19 (2020)
21. 2019
    Refined mechanism of Mycoplasma mobile gliding based on structure, ATPase activity, and sialic acid binding of machinery

    Nishikawa MS†, Nakane D†, Toyonaga T, Kawamoto A, Kato T, Namba K, Miyata M*

    mBio 10: e02846-19 (2019)
22. 2019
    Motor torque measurement of Halobacterium salinarum archaellar suggests a general model for ATP-driven rotary motors

    Iwata S, Kinosita Y, Uchida N, Nakane D*, Nishizaka T*

    Communications Biology 2: 199 (2019)
23. 2019
    Characterization of zoospore type IV pili in Actinoplanes missouriensis

    Kimura T, Tezuka T*, Nakane D, Nishizaka T, Aizawa SI, and Ohnishi Y*

    Journal of Bacteriology 201: e00746-18 (2019)
24. 2019
    Single-molecule pull-out manipulation of the shaft of the rotary motor F1-ATPase

    Naito T, Masaike T*, Nakane D, Sugawa M, Okada K, Nishizaka T*

    Scientific Reports 9: 7451 (2019)
25. 2018
    Three-dimensional tracking of microbeads attached to the tip of single isolated tracheal cilia beating under external load

    Katoh TA, Ikegami K, Uchida N, Iwase T, Nakane D, Masaike T, Setou M, Nishizaka T*

    Scientific Reports 8: 15562 (2018)
26. 2018
    Immunoglobulin-like domains of the cargo proteins are essential for protein stability during secretion by the type IX secretion system

    Sato K*, Kakuda S, Yukitake H, Kondo Y, Shoji M, Takebe K, Narita Y, Naito M, Nakane D, Abiko Y, Hiratsuka K, Suzuki M, Nakayama K

    Molecular Microbiology 110: 64-81 (2018)
27. 2018
    Identification of a major glucose transporter in Flavobacterium johnsoniae: Inhibition of F. johnsoniae colony spreading by glucose uptake

    Imamura K, Sato K, Narita Y, Kondo Y, Nakane D, Naito M, Fujiwara T, Nakayama K*

    Microbiology and Immunology 62: 507-516 (2018)
28. 2018
    Unforeseen swimming and gliding mode of an insect gut symbiont, Burkholderia sp. RPE64, with wrapping of the flagella around its cell body

    Kinosita Y*, Kikuchi Y*, Mikami N, Nakane D, and Nishizaka T

    The ISME Journal 12: 838-848 (2018)
29. 2017
    Asymmetric distribution of type IV pili triggered by directional light in unicellular cyanobacteria

    Nakane D, and Nishizaka T*

    Proc. Natl. Acad. Sci. USA 114: 6593-98 (2017)
30. 2017
    Chemotaxis without conventional two-component system, based on cell polarity and aerobic conditions in helicity-switching swimming of Spiroplasma eriocheiris

    Liu P, Zheng H, Meng Q, Terahara N, Gu W, Wang S, Zhao G, Nakane D, Wang W, Miyata M*

    Frontiers in Microbiology 8: 58 (2017)
31. 2016
    Direct observation of rotation and steps of the archaellum of swimming halophilic archaea

    Kinosita Y, Uchida N*, Nakane D and Nishizaka T*

    Nature Microbiology 1: 16148 (2016)
32. 2016
    Directed binding of gliding bacterium, Mycoplasma mobile, shown by detachment force and bond lifetime

    Tanaka A, Nakane D, Mizutani M, Nishizaka T and Miyata M*

    mBio 7: e00455-16 (2016)
33. 2015
    Systematic structural analyses of attachment organelle in Mycoplasma pneumoniae

    Nakane D†, Kenri T†, Matsuo L and Miyata M*

    PLOS Pathogens 11: e1005299 (2015)
34. 2015
    Bacteriocin protein BacL1 of Enterococcus faecalis targets cell division loci and specifically recognizes L-Ala2-crossbridged peptidoglycan

    Kurushima J, Nakane D, Nishizaka T and Tomita H*

    Journal of Bacteriology 197: 286-95 (2015)
35. 2014
    Unitary step of gliding machinery in Mycoplasma mobile

    Kinosita Y†, Nakane D†, Sugawa M, Masaike T, Mizutani K, Miyata M*, Nishizaka T*

    Proc. Natl. Acad. Sci. USA 111: 8601-06 (2014)
36. 2014
    Lack of a surface layer in Tannerella forsythia mutants deficient in the type IX secretion system

    Narita Y, Sato K, Yukitake H, Shoji M, Nakane D, Nagano K, Yoshimura F, Naito M, Nakayama K*

    Microbiology 160: 2295-303 (2014)
37. 2013
    Physical basis for the emergence of motility driven by cell-surface flow

    Wada H*, Nakane D and Chen HY

    Physical Review Letters 111: 248102 (2013)
38. 2013
    Helical flow of surface protein required for bacterial gliding motility

    Nakane D, Sato K, Wada H, McBride MJ* and Nakayama K*

    Proc. Natl. Acad. Sci. USA 110: 11145-11150 (2013)
39. 2013
    Binding in Mycoplasma mobile and Mycoplasma pneumoniae gliding analyzed through inhibition by synthesized sialylated compounds

    Kasai T, Nakane D, Ishida H, Ando H, Kiso M and Miyata M*

    Journal of Bacteriology 195: 429-435 (2013)
40. 2013
    Decoding system for the AUA codon by tRNAIle with the UAU anticodo n in Mycoplasma mobile

    Taniguchi T, Miyauchi K, Nakane D, Miyata M, Muto A, Nishimura S and Suzuki T*

    Nucleic Acids Research 41: 2621-2631 (2013)
41. 2012
    Flavobacterium johnsoniae RemA is a mobile cell-surface lectin involved in gliding

    Shrivastava A, Rhodes RG, Pochiraju S, Nakane D and McBride MJ*

    Journal of Bacteriology 194: 3678-3688 (2012)
42. 2012
    Mycoplasma mobile cells elongated by detergent and their pivoting movements in gliding

    Nakane D and Miyata M*

    Journal of Bacteriology 194: 122-130 (2012)
43. 2012
    "Mycoplasmal antigen modulation," a novel surface variation suggested for a lipoprotein specifically localized on Mycoplasma mobile

    Wu HN, Kawaguchi C, Nakane D and Miyata M*

    Current Microbiolgy 64: 433-440 (2012)
44. 2012
    Rapid imaging of mycoplasma in solution using atmospheric scanning electron microscopy

    Sato C*, Manaka S, Nakane D, Nishiyama H, Suga M, Nishizaka T, Miyata M, Maruyama Y

    Biochemical and Biophysical Research Communications 417: 1213-1218 (2012)
45. 2011
    Isolation and characterization of P1 adhesin, a leg protein of the gliding bacterium Mycoplasma pneumoniae

    Nakane D, Adan-Kubo J, Kenri T, Miyata M*

    Journal of Bacteriology 193: 715-722 (2011)
46. 2009
    Cytoskeletal asymmetrical-dumbbell structure of a gliding mycoplasma, Mycoplasma gallisepticum, revealed by negative-staining electron microscopy

    Nakane D and Miyata M*

    Journal of Bacteriology 191: 3256-3264 (2009)
47. 2009
    Regions on Gli349 and Gli521 protein molecules directly involved in movements of Mycoplasma mobile gliding machinery suggested by inhibitory antibodies and mutants

    Uenoyama A, Seto S, Nakane D and Miyata M*

    Journal of Bacteriology 191: 1982-1985 (2009)
48. 2007
    Cytoskeletal "jellyfish" structure of Mycoplasma mobile

    Nakane D and Miyata M*

    Proc. Natl. Acad. Sci. USA 104: 19518-19523 (2007)

Reviews & book chapters

1. 2025
   微生物は極限環境でかしこく行動するのかな？

   上村直輝、中根大介*

   極限環境生物学会誌 23: 19-24 (2025)
2. 2025
   Type IV pili in thermophilic bacteria: mechanisms and ecological implications

   Uemura NA and Nakane D*

   Biomolecules 15: 459 (2025)
3. 2024
   Overview of the Bacterial/Archaeal Supermolecular Assembly session at the 21st IUPAB and the 62nd Biophysics Society of Japan joint congress

   Nakane D and Tulum I*

   Biophysical Review 16: 533-536. (2024)
4. 2023
   Frontiers of microbial movement research

   Minamino T*, Nakane D, Nakamura S, Kiyama H, Morimoto YV, Miyata M

   Biophysics and Physicobiology 20: e200033 (2023)
5. 2023
   Rheotaxis in Mycoplasma gliding

   Nakane D*

   Microbiology and Immunology 67: 389-395 (2023)
6. 2023
   Live cell imaging of the twitching motility of cyanobacteria by high-resolution microscopy

   Nakane D*

   Methods in Molecular Biology [Bacterial and Archaeal Motility], Chapter 20: 255-263 (2023)
7. 2023
   Isolation and visualization of gliding motility machinery in Bacteroidota

   Shibata S*, and Nakane D

   Methods in Molecular Biology [Bacterial and Archaeal Motility], Chapter 21: 267-276 (2023)
8. 2023
   Live cell imaging of gliding motility of Bacteroidetes under high-resolution microscopy

   Nakane D*, and Shibata S

   Methods in Molecular Biology [Bacterial and Archaeal Motility], Chapter 22: 277-286 (2023)
9. 2023
   Swimming motility assays of Spiroplasma

   Nakane D*.

   Methods in Molecular Biology [Bacterial and Archaeal Motility], Chapter 31: 373-381 (2023)
10. 2022
    Design principles of the rotary type 9 secretion system

    Trivedi A, Gosai J, Nakane D, Shrivastava A

    Frontiers in Microbiology 13: 845563 (2022)
11. 2021
    精密3次元位置検出が拓く多様な分子モーター研究への新しいアプローチ

    西坂崇之, 加藤孝信, 中根大介

    生物物理 31: 10-16 (2021)
12. 2020
    動きの可視化から紐解くバクテリアの新しい世界

    中根大介*, 西坂崇之

    日本乳酸菌学会誌 31: 10-16 (2020)
13. 2020
    Tree of motility - A proposed history of motility systems in the tree of life

    Miyata M*, Robinson RC*, Uyeda TQP*, Fukumori Y, Fukushima SI, Haruta S, Homma M, Inaba K, Ito M, Kaito C, Kato K, Kenri T, Kinosita Y, Kojima S, Minamino T, Mori H, Nakamura S, Nakane D, Nakayama K, Nishiyama M, Shibata S, Shimabukuro K, Tamakoshi M, Taoka A, Tashiro Y, Tulum I, Wada H, Wakabayashi KI

    Genes to Cells 25: 6-21 (2020)
14. 2019
    Insights into the mechanism of ATP-driven rotary motors from direct torque measurement

    Nishizaka T*, Masaike T, and Nakane D

    Biophysical Reviews 11: 653-657 (2019)
15. 2019
    Preparation of Actinoplanes missouriensis zoospores and assay for their adherence to solid surfaces

    Tezuka T, Nakane D, Kimura T, Ohnishi Y

    Bio-protocol 9: e3458 (2019)
16. 2018
    光に応答したバクテリアIV型線毛の動的制御

    中根大介*, 西坂崇之

    生物物理 58: 207-208 (2018)
17. 2018
    シアノバクテリアの運動

    中根大介*, 西坂崇之

    生物工学会誌 96: 244-247 (2018)
18. 2015
    Flavobacterium gliding motility and the type IX secretion system

    McBride MJ* and Nakane D

    Current Opinion in Microbiology 28: 72-77 (2015)
19. 2015
    細菌の滑走運動メカニズムに関する研究

    中根大介*

    日本細菌学雑誌 70: 375-82 (2015)
20. 2015
    バクテリア滑走運動の新しいメカニズム ~戦車のような仕組みで動くバクテリア~

    中根大介*, 中山浩次, 西坂崇之

    生物と化学 53: 215-216 (2015)
21. 2014
    戦車のような仕組みで動くバクテリア

    中根大介*, 中山浩次, 西坂崇之

    生物物理 54: 269-270 (2014)
22. 2010
    病原細菌, マイコプラズマのユニークな滑走運動を支える細胞骨格

    宮田真人*, 中根大介

    生物の科学 遺伝 64: 47-51 (2010)
23. 2009
    Mycoplasma pneumoniae の滑走運動

    宮田真人*, 中根大介

    生体の科学 60: 98-102 (2009)